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Histologist

Location: Amsterdam
Start: Preferably asap (aim is SEP/OCT)


Purpose of the role
The ideal candidate should have a deep knowledge of histology and imaging of CNS directed gene therapy. The successful candidate will have proven extensive hands-on experience in manual and automated staining, whole-slide imaging and image analysis. This is a full-time position (40 hours/week).


Place in the Organization
The Histology and Imaging group is a part of Research Department in Amsterdam. This group contains of 4 people, and supports all preclinical research projects by providing (a) sample preparation, staining, and (b) microscopy and imaging analysis.


The role:

· It is expected the role will entail approximately 70% lab-based work;

· Troubleshoot protocols for histological staining, performance of equipment, and improves histology workflows;

· Carry out the preparations of frozen and FFPE specimens including embedding, section cutting, routine staining;

· Perform chromogenic and fluorescent IHC, ISH, and combination IHC & ISH;

· Monitor tissue and staining quality in slides;

· Operate an autostainer and a whole slide scanner;

· Prepare documentation and reports;

· Communicate with team members, Program and Function Leads regarding histology needs, incoming studies, experiment updates, and timelines.


Your profile:

· You hold a Bachelor’s and/or Master’s degree or equivalent combination of education and experience;

· Good understanding of gene therapy read outs;

· Hands-on experience with sample preparation (cells, organs), sample fixation and prof level in sectioning with particular expertise in neuroanatomy;

· Hands-on experience with an autostainer (Leica Bond);

· Hands-on experience with a chromogenic and fluorescent IHC, ISH, combination IHC and ISH, and its troubleshooting;

· Hands-on experience with multiplex IHC (5 markers in one slide);

· Familiar with laboratory information management system is a plus;

· Experience with whole-slide imaging (Zeiss AxioScanner) is a plus;

· Experience with image analysis software (HALO, QuPath) is a plus.


Core competencies

· Able to act in a fast changing and demanding environment;

· Willing and able to demonstrate a hands-on attitude;

· Able to work independently and troubleshot;

· Able to document results accurately and be focused on output;

· You communicate well in English, both verbally and in writing.


In short:
-The ideal candidate had been working in multiple ICH and ISH projects the facility, pathology dept.

- She/he is able to understand gene therapy read-outs.

- She/he will be able to set up IHC and ISH assays in an autostainer.

- Experience with brain sectioning and staining. (1) Experience with autostainer (ideally Leica Bond RX), (2) In-situ hybridization (3) neuroanatomy

 

Are you interested? Do not hesitate and contact us directly by applying to this role!

 

Contact us

SIRE® LIFE SCIENCES B.V.

Staten Bolwerk 1
2011 MK Haarlem
The Netherlands
+31 (0) 20 658 9800

SIRE® STAFFING SOLUTIONS B.V.

Keizersgracht 440A
1016 GD Amsterdam
The Netherlands
+31 (0) 20 658 9804